Jun 13, 2023

Rotarod test

DOI
https://dx.doi.org/10.17504/protocols.io.eq2ly7b1mlx9/v1
  • 1Mitochondria and Inflammation in Neurodegenerative Diseases, DZNE, Tübingen-Germany;
  • 2Hertie Institute for Clinical Brain Research, University of Tübingen;
  • 3Neuropharmacology Laboratory, Oasi Research Institute-IRCCS, Troina, Italy;
  • 4Biomedical and Biotechnological Sciences, Pharmacology Section, University of Catania-Italy;
  • 5Institut Imagine, INSERM UMR1163 Paris Cite' University, 24 boulevard du Montparnasse, 75015, Paris, France.
carmela giachino
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DOI: https://dx.doi.org/10.17504/protocols.io.eq2ly7b1mlx9/v1
Protocol Citation: michela.deleidi , Bianca Marchetti, Federico Bertoli, Carmela Giachino, carmela.giachino 2023. Rotarod test. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly7b1mlx9/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 31, 2023
Last Modified: June 13, 2023
Protocol  Integer ID: 79823
Keywords: ROTAROD, rotarod test rotarod test, aging process, effect of lps treatment, motor performance, lps treatment, genotype
Funders Acknowledgements:
ASAP-Aligning Science Across Parkinson’s
Grant ID: ASAP-000420
Abstract
Rotarod test was used to evaluate the effect of LPS treatment on motor performance during aging process between genotypes.
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ROTAROD
12h 5m
To test motor performance, we used the accelerated Rotarod (five-lane accelerating rotarod; Ugo Basile, Comerio, Italy), a motor behavioral test widely used to assess motor deficit in neurodegenerative disease models in rodents. Mice have to keep their balance on a horizontal rotating rod (diameter, 3 cm) and rotation speed is progressively increased every 30 sec by 4 rpm.


Before the test, WT and G2019S within the different treatment groups were housed five per cage and acclimated to a 12h shift in light/dark cycle so that the exercise occurred during the animal normal wake period.

To start the trials, the mice (five mice are tested at the same time) are placed on a rotating rod; when the mice fall down or when 5 min are completed, a switch is activated that automatically stops a timer. On the day of testing, the mice perform 5 trials, separated by an interval of 30 min between each trial.


The Rotarod performance was assessed at day -7, 0 days, and during NaCL/LPS injection paradigm, at monthly intervals, for the determination of motor deficit (Supplementary Figure 1).