Apr 17, 2026

RNASEQ protocol at Plasmidsaurus

DOI
https://dx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1
  • 1University of Washington
  • IGVF
Beth Martin
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DOI: https://dx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1
Protocol Citation: Beth Martin 2026. RNASEQ protocol at Plasmidsaurus. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 16, 2026
Last Modified: April 17, 2026
Protocol  Integer ID: 315221
Keywords: rnaseq protocol at plasmidsaurus rnaseq protocol, plasmidsaurus rnaseq protocol, rnaseq protocol, plasmidsaurus, protocol
Abstract
RNASEQ protocol at Plasmidsaurus
RNASEQ protocol at Plasmidsaurus
Plasmidsaurus RNASEQ Library Preparation
https://plasmidsaurus.com/faq/rna#rna-library-prep
Poly-A mRNA capture and reverse transcription for cDNA synthesis: oligo-dT primer to capture just the mRNA (contains sample barcode, UMI, and Read 1 sequences).
Second strand synthesis and tagmentation: double stranded-cDNA is generated. Tagmentation creates fragments and incorporates Read 2 sequence.
Illumina library amplification: adds unique dual indices (UDI = i5, i7) and P5/P7 sequences.