Jul 25, 2023

RNAlater preparation 

DOI
https://dx.doi.org/10.17504/protocols.io.eq2lyjn6wlx9/v1
  • 1Kaohsiung Medical University
Tsu-Chun Hung
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DOI: https://dx.doi.org/10.17504/protocols.io.eq2lyjn6wlx9/v1
Protocol Citation: Tsu-Chun Hung 2023. RNAlater preparation . protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyjn6wlx9/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 25, 2023
Last Modified: September 13, 2023
Protocol  Integer ID: 85447
Keywords: rnalater preparation, preparation
Abstract
RNAlater preparation
DEPC-treated water prepartion
Prepare 0.1% DEPC-treated water and autocleave it
AB
ComponentVolume
DEPC0.5 mL
DDW500 mL

RNAlater preparation
Add these component in 1L bottle in order
AB
ComponentVolume
Ammonium sulfate350 g
DEPC-treated water467.5 mL
1M tri-sodium citrate12.5 mL
0.5M EDTA20 mL

Put the bottle on the stirring hot plate, stir till the mixture clear

Note
Remember to loose the lid when heating


After cool down, add 8.96 mL 1M sulfuric acid to adjust pH to 5.2