Protocol for obtaining high quality RNA from yeast using acid-phenol lysis at 65°C. Modifed from DOI: 10.1016/S0076-6879(02)50976-9 to use 10-15 ml of yeast culture. Expected yield of 100-200 μg of RNA. For qPCR or RNA-seq, we recommend DNase-treatment and further purification (e.g. Zymo RNA Purification Kit (R1057)).