Implemented by: Falicia Goh and Neil ClarkeThis RNA isolation method is a combination and modification of the hot acid phenol method (protocol 14) and that described by Asif et al6. This method was used for two taxa (P. cruentum and B. braunii). This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https:\/\/www.protocols.io\/view\/rna-isolation-from-plant-tissue-439gyr6) 6 Asif, M.H., Dhawan, P. & Nath, P. A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Molecular Biology Reporter 18, 109\u2010115 (2000).