Implemented by: Sarah Covshoff, Rowan Sage and Julian HibberdThis RNA isolation method is a multi-component method involving an initial extraction by hotacid phenol and then a purification and DNase treatment using the RNeasy Mini Kit by Qiagen.The method described below is a modification of a method described by van Tunen et al.3.This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https:\/\/www.protocols.io\/view\/rna-isolation-from-plant-tissue-439gyr6) 3 van Tunen, A.J. et al. Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light\u2010 regulated and differential expression of flavonoid genes. The EMBO Journal 7, 1257\u20101263 (1988).