Aug 21, 2019
RNA Isolation from Plant Tissue Protocol 1: Qiagen RNeasy Plant Mini Kit
Peer-reviewed method
- Jim Leebens-Mack,
- Charlotte Carrigan
- GigaScience Press
External link: https://doi.org/10.1093/gigascience/giz126
Protocol Citation: Jim Leebens-Mack, Charlotte Carrigan 2019. RNA Isolation from Plant Tissue Protocol 1: Qiagen RNeasy Plant Mini Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.4vxgw7n
Manuscript citation:
Carpenter EJ, Matasci N, Ayyampalayam S, Wu S, Sun J, Yu J, Jimenez Vieira FR, Bowler C, Dorrell RG, Gitzendanner MA, Li L, Du W, K Ullrich K, Wickett NJ, Barkmann TJ, Barker MS, Leebens-Mack JH, Wong GK. Access to RNA-sequencing data from 1,173 plant species: The 1000 Plant transcriptomes initiative (1KP). Gigascience. 2019 Oct 1;8(10):giz126. doi: 10.1093/gigascience/giz126.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
These protocols were used for RNA extraction from plant tissues in order to support the One Thousand Plants initiative's work to produce RNA-Seq transcriptomes from a diverse collection of plant samples.
Created: June 28, 2019
Last Modified: August 21, 2019
Protocol Integer ID: 25239
Keywords: RNA, RNA isolation, RNA extraction, plant tissue, plant RNA
Abstract
Protocol implemented by: Jim Leebens-Mack and Charlotte Carrigan
The first protocol used for isolation of total RNA was Qiagen’s RNeasy Plant Mini Kit (Qiagen, Valencia, CA). These kits are commercially available and widely used in RNA isolation. For detailed methods associated with this kit we refer readers to the “RNeasy Mini Handbook” available for download at www.qiagen.com.
This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6)
Attachments
Materials
MATERIALS
RNeasy Plant Mini KitQiagenCatalog #74904
Safety warnings
Please see SDS (Safety Data Sheet) for hazards and safety warnings.