Protocol implemented by: Jim Leebens-Mack and Charlotte Carrigan The first protocol used for isolation of total RNA was Qiagen’s RNeasy Plant Mini Kit (Qiagen, Valencia, CA). These kits are commercially available and widely used in RNA isolation. For detailed methods associated with this kit we refer readers to the “RNeasy Mini Handbook” available for download at www.qiagen.com. This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6)