A CTAB based method for extracting RNA, which is particularly useful for tough tissues. This has been adapted from a protocol that was originally used for pine tree tissue, which is difficult due to the high concentrations of polysaccharides, phenolics, and RNase (Chang, S., Puryear, J. & Cairney, J. Plant Mol Biol Rep (1993) 11: 113. https:\/\/doi.org\/10.1007\/BF02670468). The protocol described herein was an effective alternative to TRIzol based extractions for recovering RNA from juvenile de-etiolated tissues.