Centrifuge capable of speeds of ≥16,000 x g (e.g, Eppendorf 5424 or 5415 D), and with rotors capable of holding 1.5ml and 2.0ml microcentrifuge tubes (Eppendorf 2231000655 or equivalent)
Adjustable Calibrated Micropipettors (0.2 – 1000 µl), dedicated for RNA work only.
Filter barrier aerosol resistant micropipettor tips DNase/RNase free (0.2 – 1000 µl)
Hype-Wipe Disinfecting Towelettes (Fisher Scientific 14-412-56 or equivalent)
DNase/RNase-free microcentrifuge tubes 1.5 mL, non-stick, low retention, siliconized (Life Technologies AM12450) OR DNase/RNase-free microcentrifuge tubes 2.0 mL, non-stick, low retention, siliconized (Life Technologies AM12475)
Qiagen RNeasy Mini Kit (Qiagen 74104) OR
OneStep™ PCR Inhibitor Removal Kit (Zymo Research, D6030)
Centrifuge capable of speeds of ≥8,000 x g (e.g, ThermoFisher RC6), and with rotors capable of holding 15ml tubes (ThermoFisher Scientific, 36-101-0816, or equivalent)
Adjustable Calibrated Micropipettors (0.2 – 1000 µl), dedicated for RNA work only.
Filter barrier aerosol resistant micropipettor tips DNase/RNase free (0.2 – 1000 µl)
Hype-Wipe Disinfecting Towelettes (Fisher Scientific 14-412-56 or equivalent)
DNase/RNase-free microcentrifuge tubes 1.5 mL, non-stick, low retention, siliconized (Life Technologies AM12450) OR DNase/RNase-free microcentrifuge tubes 2.0 mL, non-stick, low retention, siliconized (Life Technologies AM12475)
5 ml low bind centrifuge tubes (Eppendorf 0030122348)
5 ml Stripettes (Fisher Scientific 07-200-9, or equivalent)
Pipet Boy ACU (Integra Biosciences 155000, or equivalent)
15 ml conical tubes (Fisher Scientific 14-959-49D, or equivalent)
Qiagen RNeasy Midi Kit (Qiagen 75144)
OneStep™ PCR Inhibitor Removal Kit (Zymo Research, D6030)