May 14, 2024

RNA extraction and PCR protocol

DOI
https://dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
  • Enrico Bagnoli1,2,
  • Miratul Muqit1,2
  • 1Aligning Science Across Parkinson’s;
  • 2Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Francesca Tonelli
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
Collection Citation: Enrico Bagnoli, Miratul Muqit 2024. RNA extraction and PCR protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 23, 2024
Last Modified: May 14, 2024
Collection  Integer ID: 98709
Keywords: ASAPCRN, extraction of rna, rna extraction, pcr protocol, pcr protocol this protocol, pcr analysis, pcr, rna, extraction, protocol detail
Funders Acknowledgements:
ASAP
Grant ID: ASAP-000463
Abstract
This protocol details the extraction of RNA and PCR analysis.
Files
Protocol
Name
RNA extraction using the PureLink® RNA Mini Kit
Version 1
Created by
Image of Francesca Tonelli, MRC-PPU at The University of Dundee
Francesca TonelliMRC-PPU at The University of Dundee
Protocol
Name
cDNA synthesis using the QuantiTect Reverse transcription kit
Version 1
Created by
Image of Francesca Tonelli, MRC-PPU at The University of Dundee
Francesca TonelliMRC-PPU at The University of Dundee
Protocol
Name
PCR and analysis
Version 1
Created by
Image of Francesca Tonelli, MRC-PPU at The University of Dundee
Francesca TonelliMRC-PPU at The University of Dundee