License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: December 05, 2024
Last Modified: December 18, 2024
Collection Integer ID: 116034
Keywords: Genomic DNA, RNeasy, Code analysis, dna extraction from formalin, tissue samples rna, rna isolation with adequate quality, dna extraction, residual dna, rna with sufficient quality, rna isolation, degradation of rna, rna, tissue sample, formalin fixation, sequencing data, dna
Funders Acknowledgements:
National Institute of Dental and Craniofacial Research
Grant ID: R21DE032344
Abstract
RNA and DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples can be a challenging process due to the cross-linking and degradation of RNA/DNA caused by formalin fixation. However, we demonstrated with the right protocol, it is possible to extract RNA with sufficient quality to generate reliable sequencing data as well as residual DNA recovered during RNA isolation with adequate quality for SNP array platforms.
