Sep 22, 2019

Public workspaceRestriction Enzyme Digestion (Protocol for NEB CutSmart® Buffer)

DOI
dx.doi.org/10.17504/protocols.io.7jhhkj6
  • 1Wageningen University
  • iGEM Wageningen 2019
Alba Balletbó
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DOI: dx.doi.org/10.17504/protocols.io.7jhhkj6
Protocol Citation: Alba Balletbó 2019. Restriction Enzyme Digestion (Protocol for NEB CutSmart® Buffer). protocols.io https://dx.doi.org/10.17504/protocols.io.7jhhkj6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 22, 2019
Last Modified: September 22, 2019
Protocol Integer ID: 27977
Abstract
This protocol is used to check if the two selected restriction enzymes can perform effective catalysis in the same solution.
Materials
MATERIALS
ReagentCutSmart Buffer - 5.0 mlNew England BiolabsCatalog #B7204S
Mix DNA solution with the suitable amount of the master mix. Reaction system for a total volume of 25μL:
DNA solution20 μL
10x NEB CutSmart® Buffer2.75 μL
Restriction enzyme1.0 μL
ddH2O1.25 μL
Total Volume25 μL

Mix gently in the eppendorf tube.
Incubate at 37ºC for 20 minutes.