Feb 20, 2022

Public workspaceRemoval of Single-Stranded Extension using Mung Bean Nuclease (M0250) V.2

DOI
dx.doi.org/10.17504/protocols.io.bb2riqd6
  • 1New England Biolabs
  • New England Biolabs (NEB)
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DOI: dx.doi.org/10.17504/protocols.io.bb2riqd6
External link: https://www.neb.com/protocols/0001/01/01/removal-of-single-stranded-extension
Protocol Citation: New England Biolabs 2022. Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250). protocols.io https://dx.doi.org/10.17504/protocols.io.bb2riqd6Version created by New England Biolabs
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: January 31, 2020
Last Modified: February 20, 2022
Protocol Integer ID: 32561
Keywords: single extension, removing single stranded extensions, mung bean nuclease
Abstract
This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250).
Materials
MATERIALS
ReagentMung Bean Nuclease - 1,500 unitsNew England BiolabsCatalog #M0250S
Safety warnings
Please see SDS (Safety Data Sheet) for hazards and safety warnings.
Suspend DNA (Concentration0.1 μg/μl ) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1.1, 2.1 or CutSmart®.
Add 1.0 unit of Mung Bean Nuclease per μg DNA.
Pipetting
Incubate at Temperature30 °C for Duration00:30:00 .
Incubation
Inactivate the enzyme by phenol/chloroform extraction or by addition of SDS to 0.01%.
Recover the DNA by ethanol precipitation.