Mar 06, 2020
- 1Hainan University
External link: https://doi.org/10.1371/journal.pone.0234435
Protocol Citation: Jing Xu 2020. Reducing Power. protocols.io https://dx.doi.org/10.17504/protocols.io.bda2i2ge
Manuscript citation:
Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 05, 2020
Last Modified: March 06, 2020
Protocol Integer ID: 33850
Abstract
The reducing power of samples were assayed using the Guo et al.1 ml of the extractwas added to 2.5 ml of phosphate buffer (0.2 M, pH 6.6) and 2.5 ml of 1% potassium ferricyanide.After 20 min, 2.5 ml of 10% trichloroacetic acid (TCA)was added, followed by centrifugation at 3000 rpm for 10 min. The upper layer (2.5 ml) was mixed with 2.5 ml of distilled water and 0.5 ml of 0.1% ferric chloride, after 10 min, the absorbance was measured at 700 nm. EC50 was calculated from the graph of an inhibition percentageagainst extract concentration.