This document describes the standard operating procedure for the application of the real time Mu-RPA assay with Exo probe system. The assay detects M. ulcerans DNA (IS2404) from clinical sample or culture suspension. The assay consists of recombinase polymerase amplification TwistDx Exo kit procedure. The reaction mix must be prepared in an environment free of DNA amplicons. Personal protective clothing (i.e. lab coats, gloves) must be used throughout the process.