Nov 20, 2025
Recipe 4 for Avibacterium paragallinarum Strains from Yangzhou University
- wujunfeng 1
- 1Nanjing University of Posts and Telecommunications
Protocol Citation: wujunfeng 2025. Recipe 4 for Avibacterium paragallinarum Strains from Yangzhou University. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvodz2xg4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233040
Keywords: avibacterium paragallinarum strain, deep16sculture ai framework for avibacterium paragallinarum strain, yangzhou university this recipe, recipe, yangzhou university, yangzhou, deep16sculture ai framework
Abstract
This recipe was generated by our Deep16SCulture AI framework for Avibacterium paragallinarum Strains from Yangzhou University.
Materials
- Brain heart infusion agar: 52,000 mg
- Horse blood: 100 ml
- Formate solution: 100 ml (optional)
- Fumaric acid solution: 100 ml (optional)
- NAD: 10 mg (added)
- Hemin: 5 mg (added)
- Water: 1,000 ml
Troubleshooting
Safety warnings
Optional: Formate/fumaric acid solutions. The absence of formate and fumaric acid might slightly reduce growth efficiency under anaerobic conditions, but this typically wouldn’t prevent successful cultivation.
Before start
Notes: Blood provides X/V factors; incubate anaerobically.
Prepare the following materials for the cultivation of Avibacterium paragallinarum strains:
- Brain heart infusion agar: 52,000 mg
- Horse blood: 100 ml
- Formate solution: 100 ml (optional)
- Fumaric acid solution: 100 ml (optional)
- NAD: 10 mg (added)
- Hemin: 5 mg (added)
- Water: 1,000 ml
Optional: Formate/fumaric acid solutions. The absence of formate and fumaric acid might slightly reduce growth efficiency under anaerobic conditions, but this typically wouldn’t prevent successful cultivation.
Notes: Blood provides X/V factors; incubate anaerobically.