Nov 20, 2025

Public workspaceRecipe 2 for Avibacterium paragallinarum Strains from Yangzhou

  • wujunfeng 1
  • 1Nanjing University of Posts and Telecommunications
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Protocol Citationwujunfeng 2025. Recipe 2 for Avibacterium paragallinarum Strains from Yangzhou. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvje4q5gk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233039
Keywords: avibacterium paragallinarum strain, deep16sculture ai framework for avibacterium paragallinarum strain, yangzhou this recipe, recipe, yangzhou, yangzhou university
Abstract
This recipe was generated by our Deep16SCulture AI framework for Avibacterium paragallinarum Strains from Yangzhou University.
Materials
Original:
- Agar: 15,000 mg
- Trypticase soy broth: 30,000 mg
- Yeast extract: 3,000 mg
- Water: 1,000 ml

Improvements:
- Added: 100 ml horse blood (V factor).
- Optional: Yeast extract (if blood is fresh).
- Notes: Supplement with 0.1% NAD if blood is heat-treated.
Troubleshooting
Add 15,000 mg of agar to the mixture.
Add 30,000 mg of trypticase soy broth.
Add 3,000 mg of yeast extract.
Add 1,000 ml of water.
Add 100 ml of horse blood (V factor).
Optionally, add yeast extract if the blood is fresh.
Supplement with 0.1% NAD if the blood is heat-treated.