Nov 20, 2025
Recipe 1 for Avibacterium paragallinarum Strains from Yangzhou
- wujunfeng 1
- 1Nanjing University of Posts and Telecommunications
Protocol Citation: wujunfeng 2025. Recipe 1 for Avibacterium paragallinarum Strains from Yangzhou. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpmn5pgzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 20, 2025
Last Modified: November 20, 2025
Protocol Integer ID: 233037
Keywords: avibacterium paragallinarum strain, deep16sculture ai framework for avibacterium paragallinarum strain, yangzhou this recipe, yangzhou, yangzhou university, recipe
Abstract
This recipe was generated by our Deep16SCulture AI framework for Avibacterium paragallinarum Strains from Yangzhou University.
Materials
- Columbia agar: 39,000 mg
- Horse blood: 100 ml (critical for XV factors)
- NAD (V factor): 10 mg (added)
- Hemin (X factor): 5 mg (added)
- Water: 1,000 ml
Troubleshooting
Before start
- Add horse blood and NAD/Hemin post-autoclaving (50°C).
- Incubate at 37°C with 5% CO₂.
Add horse blood and NAD/Hemin post-autoclaving (50°C).
Incubate at 37°C with 5% CO₂.