Sep 02, 2020
RAPID RNA Extraction
- Noah Toppings1
- 1University of Calgary
- XPRIZE Rapid Covid Testing
Protocol Citation: Noah Toppings 2020. RAPID RNA Extraction. protocols.io https://dx.doi.org/10.17504/protocols.io.bkp8kvrw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 02, 2020
Last Modified: September 02, 2020
Protocol Integer ID: 41440
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Add 140 µL saliva to 560 µL lysis buffer.
Shake the tubes violently by hand.
Incubate at 61 ºC for 5 min.
Apply entire 700 µL to spin column.
Spin at 8 kRCF for 1 min.
Remove flowthrough with a pipette.
Add 500 µL wash 1 to the column.
Spin at 8 kRCF for 1 min.
Remove flowthrough with a pipette.
Add 500 µL wash 2.
Spin at 8 kRCF for 2 min.
Transfer column to a new tube and add 50 µL elution buffer.
Spin at 8k RCF for 1 min.
Add to LAMP reactions.