May 10, 2025

RAISINs (RNA-seq for Profiling Intact Nuclei with Ribosome-bound mRNA) Nuclei Isolation from Mouse CNS Tissue Protocol V.2

  • Allen Institute1
  • 1Allen Institute
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Protocol CitationAllen Institute 2025. RAISINs (RNA-seq for Profiling Intact Nuclei with Ribosome-bound mRNA) Nuclei Isolation from Mouse CNS Tissue Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l22n5pl1y/v2Version created by Allen Institute
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 10, 2025
Last Modified: May 10, 2025
Protocol  Integer ID: 218018
Keywords: nuclei isolation from mouse cns tissue protocol, nuclei from mouse cns tissue, seq for profiling intact nuclei, nuclei isolation, profiling intact nuclei, mouse cns tissue protocol, rna, mouse cns tissue, ribosome, nuclei, raisin, bound mrna, mrna
Abstract
This protocol is used to isolate nuclei from mouse CNS tissue.