The protocol was developed for observing salt-induced changes in Root System Architecture of Arabidopsis and other small plants. The seeds are germinated in control conditions, and exposed to stress 4 days after germination. As the agar plates are transparent, this system provides quick, easy and non-destructive method to observe development of roots. This protocol was used to produced the data in two manuscripts from our lab - Julkowska et al., 2014 Plant Physiology and Julkowska et al., 2017 Plant Cell.