Protocol Citation: vito 2020. Qbiotix SARS-CoV-2 protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bkvckw2w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 05, 2020
Last Modified: September 05, 2020
Protocol Integer ID: 41604
Abstract
Qbiotix has developed novel nucleic acid assays and sample preparation procedures to improve global microbial detection standards and rapidly/inexpensively test high volume of samples. Qbiotix aims to enable countries to test their entire populations, their food, their waters with millions of tests per day on a regular basis.
The assays are isothermal and homogeneous. Thermocycling is not required and amplification is non-enzymatic removing the need for complex instrumentation and reliance on variable quality enzymes. Critically we have removed the need for complex nucleic acid preparation, integrated the assay into the collection/transport solution and significantly reduced reliance on a complex supply chain. The assays remove a good proportion of the constraints of PCR which heretofore have inhibited the number of people being testing for SARS-CoV-2 in the US and globally.
Guidelines
The test should be run in a standard laboratory
Before start
Need to have kits and saliva collection devices provided by Qbiotix. Also need to have calibrated pipettes, tips, a magnetic separator and a microtiter plate analyzer.
1- collect saliva vial1 mL in a collection deviprovided by Qbiotix
2- open vial and scan barcode in a temperature laboratory with temperature between 18 and 25 degrees Celsius.
3- dispense 250 µL sample to single well in plate with a standard pipette
4- dispense 250 µL of magnetic bead solution in well with a standard pipette in a temperature controlled room with temperature between 18 and 25 degrees Celsius. The bead solution is supplied within a KIT provided by Qbiotix
5- incubate at controlled temperature on the plate reader 00:10:00
6- place plate on a magnetic separator 00:00:30
7- insert plate on analyzer (reading speed depends on chosen plate reader instrument). The plate reader analyzer needs to have with the ability to read fluorescence and control temperature
6- read results