Sep 03, 2025
Purification (Size selection)
Forked from Size selection (Purification)
- Yin-Tse Huang1,
- Tsu-Chun Hung1
- 1Kaohsiung Medical University
Protocol Citation: Yin-Tse Huang, Tsu-Chun Hung 2025. Purification (Size selection). protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbwrbngpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 02, 2025
Last Modified: September 03, 2025
Protocol Integer ID: 226281
Keywords: purification, size selection
Abstract
Purification (Size selection)
Troubleshooting
Prepare 20 µL sample and 9 µL magnetic beads in 1.5 mL eppendorf tube.
| A | B | C | D | |
| Component | Volume | Proportion | Note | |
| Sample | 20 μl | 1x | ||
| Magnetic beads | 9 μl | 0.45x | BeaverBeads™ DNA Select Isolation |
Mix sample and beads gently by flicking then flash spin the tube. Put on the regular rack for 5mins 00:05:00 .
5m
Transfer the tube to the magnetic rack. After most of the magnetic beads attach to the wall, remove the supernant.
Add 300 µL 80% ethanol, flip whole magnetic rack around.
3m
Repeat step 4.
Quick spin the tube, and put on the magnetic rack. Remove superfluous solution with 10 μl pipette.
Note
Caution: DO NOT let the beads crack!
Add 10 µL elution buffer. Mix gently by flicking and flash spin the tube. Incubate at 37C for 10mins 00:10:00 .
Note
DON'T put the tube on magnetic rack during waiting in this step.
10m
Transfer the tube to the magnetic rack. After all the magnetic beads attach to the wall, collect the supernant to a new DNA tube (0.5 ml skirt tube).
Ready for downstream process (e.g. 1' or 2' PCR, or library construction.