This protocol can be used to further purifiy (Kai) proteins via size exclusion chromatography using either HiPrep 16/60 Sephacryl S-300 HR column (120 ml column volume), HiPrep 16/60 Sephacryl S-200 HR (120 ml column volume) or Superdex 200 Increase 10/300 GL (24 ml column volume)