Enzyme solution for the isolation of protoplasts suitable for Arabidopsis thaliana and Nicotiana benthamiana. Adapted from Yoo et al. 2007 http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.htmlAnd excellent video from the Sheen Lab is available: https://www.youtube.com/watch?v=5-xm1EoLrW4