Feb 16, 2023
Proton Density Fat Fraction (PDFF) Measurement of Myelofibrosis in Mouse Tibia
- Thomas L Chenevert1,
- Thomas Chenevert1
- 1University of Michigan
Protocol Citation: Thomas L Chenevert, Thomas Chenevert 2023. Proton Density Fat Fraction (PDFF) Measurement of Myelofibrosis in Mouse Tibia. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwjmywlmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 16, 2023
Last Modified: February 16, 2023
Protocol Integer ID: 77086
Keywords: preclinical imaging protocol, mouse tibia, proton density fat fraction MRI, bone marrow myelofibrosis, technical repeatability, pdff measurement in mf mouse tibia, tibia bone marrow composition in mf mouse model, mf mouse model tibia, tibia bone marrow composition, mf mouse tibia, measurement of myelofibrosi, mouse tibia this document details procedure, proton density fat fraction, tibia bone marrow, pdff measurement, containing dominant red marrow, tibia section, pdff value, dominant yellow marrow, dominant red marrow, profile pdff value, mf mouse model, bone marrow, myelofibrosi, pdff, tibia, measured change in the mean pdff, analysis of pdff map, such that bone marrow, pdff map
Funders Acknowledgements:
National Institute of Health
Grant ID: U24CA237683
Disclaimer
The claims hold when:
- Scanner hardware, proton density-weighted multi gradient-echo data acquisition method and parameters, image reconstruction, and data-reduction procedures are equivalent (or superior) to those detailed in section III.
- Use of the same animal model and interventions to induce myelofibrosis are performed as detailed in section V.
- PDFF change is assessed on an individual animal basis where each animal undergoes identical procedures on the same MRI system over longitudinal timepoints.
Abstract
This document details procedures for PDFF measurement in MF mouse tibia to achieve stated performance claims.In this profile PDFF values are expressed in “% units” on a 0 to 100% scale, such that bone marrow containing dominant red marrow and dominant yellow marrow have PDFF values of 0-few% and 80-100%, respectively. Tibia bone marrow composition in MF mouse models has gradation going from proximal to distil ends of the tibia, therefore separate claims are made for volume of interest (VOI) analysis of PDFF maps for each of three distinct sections along the length of the tibia (see Figure 1):
Section 1 (proximal)º VOI (~4-5mm3) within 9mm of proximal end of tibia
Section 2 (transition)º VOI (~0.4-0.5mm3) from 10 to 12mm of proximal end of tibia
Section 3 (distil)º VOI (~0.1-0.2mm3) from 13 to 14mm of proximal end of tibia
Claim 1:A measured change in the mean PDFF in Section 1 VOI of MF mouse model tibia that exceeds ±1.6% indicates a true biological change has occurred in the tibia bone marrow with 95% confidence.
Claim 2:A measured change in the mean PDFF in Section 2 VOI of MF mouse model tibia that exceeds ±15.5% indicates a true biological change has occurred in the tibia bone marrow with 95% confidence
Claim 3:A measured change in the mean PDFF in Section 3 VOI of MF mouse model tibia that exceeds ±25.5% indicates a true biological change has occurred in the tibia bone marrow with 95% confidence
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