Feb 07, 2024
Version 2
Protocols from Kilfeather, Khoo et al., 2024 V.2
- 1University of Oxford
Collection Citation: Peter Kilfeather, Maria Claudia Caiazza 2024. Protocols from Kilfeather, Khoo et al., 2024. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqj75kvk5/v2Version created by Peter Kilfeather
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 07, 2024
Last Modified: February 07, 2024
Collection Integer ID: 94822
Keywords: assay in kilfeather, assay, protocol collection, immunohisto, staining method, kilfeather, protocol
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-020370
Parkinson's UK
Grant ID: J-1403
Science, Technology and Innovation Commission of Shenzhen Municipality
Grant ID: JCYJ20180507183628543
Abstract
This protocol collection includes immunohisto/chemical staining methods, DAT-TRAP methods and the protocol for the Fura-2 assay in Kilfeather, Khoo et al., 2024.
Files
Protocol
Name
Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
Version 1
Created by
Peter Kilfeather
Protocol
Name
Fura-2 imaging of ionomycin response, with and without R568; a CaSR positive modulator
Version 1
Created by
Peter Kilfeather
Protocol
Name
Immunohistochemistry
Version 1
Created by
Peter Kilfeather
Protocol
Name
DAT-TRAP Protocol
Version 2
Created by
Peter Kilfeather
Protocol
Name
Stereo-seq cell type abundance analysis
Version 1
Created by
Peter Kilfeather
Protocol
Name
Differential gene expression analysis
Version 1
Created by
Peter Kilfeather
Protocol
Name
GWAS prioritization analysis
Version 1
Created by
Peter Kilfeather
Protocol
Name
Spatially variable gene detection
Version 1
Created by
Peter Kilfeather
Protocol
Name
Processing of raw Stereo-seq data, quality control and cell type identification
Version 1
Created by
Peter Kilfeather
Protocol
Name
Specificity index (pSI) calculation
Version 1
Created by
Peter Kilfeather
Protocol
Name
Differential transcript usage analysis
Version 1
Created by
Peter Kilfeather
Protocol
Name
Gene set enrichment analysis
Version 1
Created by
Peter Kilfeather
Protocol
Name
Long-read sequencing and data processing
Version 1
Created by
Peter Kilfeather