May 24, 2023

Protocols from 237 CAR-T studies in a mouse ovarian tumor model (reported in Ranoa et al JITC 2023)

  • 1University of Illinois at Urbana-Champaign
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Collection CitationDiana Rose Ranoa, Preeti Sharma, Claire P. Schane, Amber N Lewis, Edward Valdez, Edward J. Roy, David M. Kranz 2023. Protocols from 237 CAR-T studies in a mouse ovarian tumor model (reported in Ranoa et al JITC 2023). protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr4jwpgmk/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: May 09, 2023
Last Modified: May 24, 2023
Collection  Integer ID: 81610
Keywords: ovarian cancer, ovarian tumor model, immunotherapy, syngeneic mouse model, journal for immunotherapy
Funders Acknowledgements:
David M Kranz
Grant ID: NIH CA238628
Abstract
This is a collection of protocols from the manuscript: Ranoa, D. R. E.; Sharma, P.; Schane, C.P.; Lewis, A.N.; Valdez, E.; Marada, V. V. V. R.; Hager, M.; Montgomery, W.; Wolf, S. E.; Schreiber, K.; Schreiber, H.; Bailey, K.; Fan, T. M.; Hergenrother, P. J.; Roy, E. J.; Kranz, D. M. A single CAR-T cell treatment controls disseminated ovarian cancer in a syngeneic mouse model. Submitted to Journal for ImmunoTherapy of Cancer 2023; doi:10.1136/jitc-2022-006509.
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Protocol
Name
Isolation, activation, and retroviral transduction of primary T cells from murine splenocytes
Version 1
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Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
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Protocols for processing of fresh murine tissues for flow cytometry
Version 1
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Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
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Immunohistochemistry of tissue sections from formalin-fixed paraffin embedded (FFPE) samples
Version 1
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Image of Diana Rose E Ranoa, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
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SRB assay for measuring target cell killing
Version 1
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Image of Diana Rose E Ranoa, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign