Nov 24, 2020
Version 4
Protocols for "The female urinary microbiota in relation to the reproductive tract microbiota." V.4
Peer-reviewed method
- Chen Chen1,
- Lilan Hao1,
- Weixia Wei2,
- Fei Li1,
- Liju Song1,
- Xiaowei Zhang1,
- Juanjuan Dai2,
- Zhuye Jie1,
- Jiandong Li1,
- Xiaolei Song1,
- Zirong Wang1,
- Zhe Zhang1,
- Liping Zeng2,
- Hui Du2,
- Huiru Tang2,
- Tao Zhang1,
- Huanming Yang1,
- Jian Wang1,
- Susanne Brix3,
- Karsten Kristiansen1,
- Xun Xu1,
- Ruifang Wu2,
- Huijue Jia1
- 1BGI-Shenzhen, Shenzhen 518083, China.;
- 2Peking University Shenzhen Hospital, Shenzhen 518036, China.;
- 3Department of Biotechnology and Biomedicine, Technical University of Denmark, Soltofts Plads, Building 221, 2800 Kgs. Lyngby, Denmark.
- GigaScience Press
- BGI
External link: https://doi.org/10.46471/gigabyte.9
Collection Citation: Chen Chen, Lilan Hao, Weixia Wei, Fei Li, Liju Song, Xiaowei Zhang, Juanjuan Dai, Zhuye Jie, Jiandong Li, Xiaolei Song, Zirong Wang, Zhe Zhang, Liping Zeng, Hui Du, Huiru Tang, Tao Zhang, Huanming Yang, Jian Wang, Susanne Brix, Karsten Kristiansen, Xun Xu, Ruifang Wu, Huijue Jia 2020. Protocols for "The female urinary microbiota in relation to the reproductive tract microbiota.". protocols.io https://dx.doi.org/10.17504/protocols.io.bp3wmqpeVersion created by Lilan Hao
Manuscript citation:
Chen C, Hao L, Wei W, Li F, Song L, Zhang X, Dai J, Jie Z, Li J, Song X, Wang Z, Zhang Z, Zeng L, Du H, Tang H, Zhang T, Yang H, Wang J, Brix S, Kristiansen K, Xu X, Wu R, Jia H, The female urinary microbiota in relation to the reproductive tract microbiota. GigaByte. 2020.
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 24, 2020
Last Modified: November 24, 2020
Collection Integer ID: 44886
Abstract
Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples from 147 Chinese women of reproductive age and explored the nature of colonization by 16S rRNA gene amplicon sequencing, quantitative real-time PCR, and live bacteria culture. To demonstrate the utility of this approach, the intra-individual Spearman's correlation was used to explore the relationship between urine and multiple sites of the reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Streptococcus-dominated, Lactobacillus-dominated, and diverse type were the three most common urinary bacterial community types in the cohort. Detailed comparison of the urinary microbiota with multiple sites of the female reproductive tract microbiota demonstrated that the urinary microbiota were more similar to the microbiota in the cervix and uterine cavity than to those of the vagina in the same women. Our data demonstrate the potential connectivity among microbiota in the female urogenital system and provide insight and resources for exploring diseases of the urethra and genital tract.
