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Protocol CitationJosé Manuel Rodríguez Berrocal 2025. Protocolo BRADFORD. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4row8go5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 29, 2025
Last Modified: April 30, 2025
Protocol Integer ID: 192017
Keywords: Bradford, protocol
Abstract
Protocolo Bradford
En un tubo de ensayo se agregan Amount5 mL de reactivo Bradford y Amount0.1 mL de H2O. Esto corresponde al blanco.

En un tubo de ensayo se agregan Amount5 mL de reactivo Bradford y Amount0.1 mL de extracto a la concentración elegida.

Se agita y se deja reposar durante 30 minutos - 1 hora.

Se mide la espectrofotometría a 595 nm por triplicado en cubetas de 1,5 mL.