Jul 07, 2025
- Yulin Yuan1
- 1People's Hospital of Guangxi Zhuang Autonomous Region
Protocol Citation: Yulin Yuan 2025. Protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4622jgo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 05, 2025
Last Modified: July 07, 2025
Protocol Integer ID: 221789
Keywords: protocol detection, testing for p85, protocol, iga, p85, rta, testing, zta, igg, detection
Abstract
Detection (Testing for P85-Ab, VCA-IgA, Rta-IgG, Zta-IgA)
Troubleshooting
Detection
Venous blood samples of approximately 3 ml were obtained from all participants and subsequently centrifuged at 3000 rpm for 10 minutes to isolate serum. The serum samples from 100 individuals with nasopharyngeal carcinoma and 100 healthy individuals were analyzed for BNLF2b antibodies using a fully automated chemiluminescent immunoassay analyzer (Wan200+ model) manufactured by Xiamen Youmaike Medical Instrument Co., Ltd. The EB virus BNLF2b antibody assay kit was utilized as the reagent. VCA-IgA, Rta-IgG, and Zta-IgA antibody levels in the serum were quantified through the ELISA technique. All experimental steps strictly adhered to the manufacturer's guidelines.
Data Analysis
Statistical analyses were conducted utilizing SPSS software version 16.0. Graphs were generated with SPSS 16.0 and GraphPad Prism 9.5. Measurement data, determined to be non-normally distributed, were presented as medians and analyzed using the rank-sum test. Count data were represented as rates and analyzed through the chi-square test, with a significance threshold of P < 0.05.
Receiver Operating Characteristic (ROC) curves were constructed to establish the optimal cutoff value based on the maximum Youden index. Additionally, the ROC curve was utilized to calculate the area under the curve (AUC), confidence intervals (CI), sensitivity, and specificity of the diagnostic techniques.
ID Rta VCA Zta P85 是否患病 P85RtaZta RtaZta P85Zta P85Rta P85VCA P85RtaZtaVCA
1.00 0.78 2.63 7.82 292683.00 1.00 1.00000 0.99992 1.00000 1.00000 0.99993 1.00000
2.00 0.02 6.43 6.17 897814.00 1.00 1.00000 0.99549 1.00000 1.00000 1.00000 1.00000
3.00 4.89 7.35 5.64 2433873.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
4.00 1.16 0.14 5.33 2502222.00 1.00 1.00000 0.99864 1.00000 1.00000 1.00000 1.00000
5.00 0.41 2.02 4.80 115567.00 1.00 0.99795 0.98587 0.99978 0.97504 0.94914 0.99822
6.00 2.11 0.75 4.53 1511211.00 1.00 1.00000 0.99942 1.00000 1.00000 1.00000 1.00000
7.00 0.35 0.14 4.40 1419886.00 1.00 1.00000 0.97183 1.00000 1.00000 1.00000 1.00000
8.00 3.16 6.50 4.18 1533319.00 1.00 1.00000 0.99990 1.00000 1.00000 1.00000 1.00000
9.00 1.81 7.79 3.81 296200.00 1.00 1.00000 0.99692 1.00000 1.00000 1.00000 1.00000
10.00 0.41 0.14 3.76 1027884.00 1.00 1.00000 0.99990 1.00000 1.00000 1.00000 1.00000
11.00 9.92 9.61 3.60 475504.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
12.00 3.11 8.05 3.37 32716.00 1.00 0.99245 0.99963 0.90800 0.96442 0.99996 1.00000
13.00 0.31 0.14 3.33 509518.00 1.00 1.00000 0.87130 1.00000 1.00000 1.00000 1.00000
14.00 0.18 1.25 3.29 268823.00 1.00 1.00000 0.82917 1.00000 0.99999 0.99858 0.99979
15.00 6.58 7.44 3.20 1309946.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
16.00 0.45 1.53 3.18 2241028.00 1.00 1.00000 0.87997 1.00000 1.00000 1.00000 1.00000
17.00 4.69 0.68 3.17 1671730.00 1.00 1.00000 0.99998 1.00000 1.00000 1.00000 1.00000
18.00 0.10 0.15 3.15 1291089.00 1.00 1.00000 0.77003 1.00000 1.00000 1.00000 1.00000
19.00 0.24 0.68 3.09 209.00 1.00 0.22583 0.80504 0.54390 0.03891 0.04967 0.23403
20.00 11.66 9.58 0.13 618977.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
21.00 0.22 0.51 3.04 2667494.00 1.00 1.00000 0.78644 1.00000 1.00000 1.00000 1.00000
22.00 10.49 7.91 3.03 977806.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
23.00 2.25 1.26 2.95 187777.00 1.00 0.99998 0.99580 0.99997 0.99998 0.98173 0.99861
24.00 0.41 0.51 2.87 1068130.00 1.00 1.00000 0.99940 1.00000 1.00000 1.00000 1.00000
25.00 0.22 5.75 0.51 155136.00 1.00 0.99992 0.99618 0.99982 0.99989 0.99997 0.99999
26.00 3.02 3.85 2.83 1967445.00 1.00 1.00000 0.99902 1.00000 1.00000 1.00000 1.00000
27.00 0.14 3.87 2.76 49628.00 1.00 0.69377 0.67489 0.93198 0.36756 0.97929 0.99116
28.00 13.68 8.63 2.75 3259902.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
29.00 12.20 6.77 2.68 643995.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
30.00 2.14 0.99 2.61 1893628.00 1.00 1.00000 0.99138 1.00000 1.00000 1.00000 1.00000
31.00 7.06 6.92 2.56 12757.00 1.00 0.99989 1.00000 0.58431 0.99981 0.99954 0.99999
32.00 3.70 8.49 2.54 469071.00 1.00 1.00000 0.99965 1.00000 1.00000 1.00000 1.00000
33.00 1.61 9.46 2.31 2189963.00 1.00 1.00000 0.96049 1.00000 1.00000 1.00000 1.00000
34.00 8.92 7.15 0.44 100579.00 1.00 1.00000 1.00000 0.99312 1.00000 0.99998 1.00000
35.00 4.88 7.73 0.79 771708.00 1.00 1.00000 0.99995 1.00000 1.00000 1.00000 1.00000
36.00 0.73 4.56 2.08 309651.00 1.00 1.00000 0.73053 1.00000 1.00000 1.00000 1.00000
37.00 1.29 0.81 2.06 71346.00 1.00 0.95410 0.89596 0.95787 0.92834 0.38494 0.68489
38.00 0.75 5.23 0.32 46464.00 1.00 0.70421 0.68876 0.82807 0.56566 0.99749 0.99768
39.00 7.95 7.06 1.88 1446331.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
40.00 0.44 0.31 1.88 3067075.00 1.00 1.00000 0.52379 1.00000 1.00000 1.00000 1.00000
41.00 12.34 8.18 0.81 1013495.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
42.00 0.79 0.15 1.70 1848875.00 1.00 1.00000 0.64013 1.00000 1.00000 1.00000 1.00000
43.00 0.38 0.55 1.64 962401.00 1.00 1.00000 0.40661 1.00000 1.00000 1.00000 1.00000
44.00 10.96 8.35 1.63 1144174.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
45.00 1.53 8.95 1.52 110237.00 1.00 0.99254 0.86780 0.99150 0.99437 1.00000 1.00000
46.00 0.28 5.18 0.75 66054.00 1.00 0.68917 0.30568 0.90330 0.65098 0.99854 0.99739
47.00 1.92 0.15 1.46 2737991.00 1.00 1.00000 0.93215 1.00000 1.00000 1.00000 1.00000
48.00 2.31 8.29 1.44 9594.00 1.00 0.72191 0.96821 0.27277 0.66416 0.99995 0.99994
49.00 0.22 7.13 1.37 4741927.00 1.00 1.00000 0.99978 1.00000 1.00000 1.00000 1.00000
50.00 10.74 8.07 1.35 467090.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
51.00 0.39 5.56 0.68 1422881.00 1.00 1.00000 0.31534 1.00000 1.00000 1.00000 1.00000
52.00 10.85 8.47 1.34 60896.00 1.00 1.00000 1.00000 0.85833 1.00000 0.99999 1.00000
53.00 0.51 4.81 0.22 1446331.00 1.00 1.00000 0.36246 1.00000 1.00000 1.00000 1.00000
54.00 5.29 9.47 1.24 3067075.00 1.00 1.00000 0.99992 1.00000 1.00000 1.00000 1.00000
55.00 2.12 6.89 1.22 1013495.00 1.00 1.00000 0.93685 1.00000 1.00000 1.00000 1.00000
56.00 3.79 7.99 0.51 1848875.00 1.00 1.00000 0.99803 1.00000 1.00000 1.00000 1.00000
57.00 6.09 0.06 1.21 962401.00 1.00 1.00000 0.99998 1.00000 1.00000 1.00000 1.00000
58.00 3.02 3.03 0.06 1144174.00 1.00 1.00000 0.98901 1.00000 1.00000 1.00000 1.00000
59.00 0.37 6.83 1.12 110237.00 1.00 0.94489 0.24059 0.98727 0.96428 0.99998 0.99992
60.00 1.48 9.40 1.11 66054.00 1.00 0.90659 0.76572 0.86491 0.92868 1.00000 1.00000
61.00 0.29 4.54 1.11 309651.00 1.00 1.00000 0.99960 1.00000 1.00000 1.00000 1.00000
62.00 0.77 2.99 1.10 71346.00 1.00 0.81393 0.41772 0.89511 0.84806 0.95715 0.93974
63.00 1.73 8.41 1.09 46464.00 1.00 0.83869 0.84347 0.67589 0.86426 0.99999 0.99997
64.00 0.46 7.94 1.09 361.00 1.00 0.07321 0.26848 0.13505 0.05510 0.99987 0.99950
65.00 7.06 8.26 1.05 335.00 1.00 0.99927 1.00000 0.13019 0.99961 0.99992 0.99999
66.00 0.49 0.95 1.00 355.00 1.00 0.07123 0.25564 0.12464 0.05767 0.07558 0.05795
67.00 0.77 4.91 0.99 2241028.00 1.00 1.00000 0.37969 1.00000 1.00000 1.00000 1.00000
68.00 3.27 3.01 0.98 1671730.00 1.00 1.00000 0.99170 1.00000 1.00000 1.00000 1.00000
69.00 2.05 3.91 0.94 296.00 1.00 0.42403 0.89518 0.11776 0.43270 0.91279 0.90909
70.00 6.59 0.06 0.92 292683.00 1.00 1.00000 0.99999 1.00000 1.00000 1.00000 1.00000
71.00 1.10 7.84 0.92 897814.00 1.00 1.00000 0.52654 1.00000 1.00000 1.00000 1.00000
72.00 13.85 7.82 0.91 2433873.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
73.00 0.22 0.32 0.89 2502222.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
74.00 2.79 3.06 0.81 115567.00 1.00 0.99841 0.97133 0.98706 0.99946 0.99036 0.99331
75.00 2.48 0.13 0.80 1511211.00 1.00 1.00000 0.94545 1.00000 1.00000 1.00000 1.00000
76.00 0.29 9.07 0.79 1419886.00 1.00 1.00000 0.94470 1.00000 1.00000 1.00000 1.00000
77.00 5.14 7.83 0.76 1533319.00 1.00 1.00000 0.99978 1.00000 1.00000 1.00000 1.00000
78.00 1.47 5.55 0.75 193.00 1.00 0.20957 0.65559 0.09854 0.22865 0.99353 0.98564
79.00 0.29 0.36 0.71 296200.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
80.00 1.26 6.85 0.70 1511211.00 1.00 1.00000 0.53178 1.00000 1.00000 1.00000 1.00000
81.00 0.75 0.28 0.64 1419886.00 1.00 1.00000 0.26150 1.00000 1.00000 1.00000 1.00000
82.00 0.77 8.76 0.62 1785.00 1.00 0.56151 0.93939 0.09477 0.65112 0.99997 0.99992
83.00 3.48 4.23 0.59 3259902.00 1.00 1.00000 0.99067 1.00000 1.00000 1.00000 1.00000
84.00 9.37 4.44 0.56 643995.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
85.00 7.65 7.93 0.50 1893628.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
86.00 8.80 1.93 0.48 12757.00 1.00 0.99995 1.00000 0.14396 0.99999 0.37725 0.93920
87.00 0.29 0.18 0.45 469071.00 1.00 1.00000 0.86540 1.00000 1.00000 1.00000 1.00000
88.00 0.81 5.09 0.43 2189963.00 1.00 1.00000 0.22895 1.00000 1.00000 1.00000 1.00000
89.00 0.29 3.75 0.43 100579.00 1.00 0.84220 0.08994 0.95704 0.93175 0.99496 0.97979
90.00 12.56 8.05 0.40 771708.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
91.00 1.90 7.00 0.39 100579.00 1.00 0.98254 0.73767 0.95533 0.99463 0.99998 0.99992
92.00 0.77 0.05 0.37 512.00 1.00 0.35439 0.82564 0.07021 0.47992 0.01844 0.01972
93.00 1.99 7.19 0.36 1446331.00 1.00 1.00000 0.76513 1.00000 1.00000 1.00000 1.00000
94.00 11.46 5.90 0.32 3067075.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
95.00 0.77 1.95 0.27 1013495.00 1.00 1.00000 0.99999 1.00000 1.00000 1.00000 1.00000
96.00 5.47 7.34 0.25 1848875.00 1.00 1.00000 0.99977 1.00000 1.00000 1.00000 1.00000
97.00 15.05 6.33 0.25 962401.00 1.00 1.00000 1.00000 1.00000 1.00000 1.00000 1.00000
98.00 3.69 2.60 0.17 1144174.00 1.00 1.00000 0.98905 1.00000 1.00000 1.00000 1.00000
99.00 0.29 10.63 0.14 110237.00 1.00 1.00000 1.00000 0.96612 1.00000 1.00000 1.00000
100.00 0.29 6.85 0.10 66054.00 1.00 0.86526 0.57915 0.69544 0.95347 0.99991 0.99957
101.00 0.72 2.89 0.15 213.00 0.00 0.05064 0.14080 0.05599 0.08097 0.66214 0.33541
102.00 0.59 2.23 0.62 239.00 0.00 0.06082 0.19694 0.08757 0.06671 0.39924 0.22403
103.00 0.53 1.45 0.08 223.00 0.00 0.03662 0.09017 0.05236 0.06086 0.15601 0.04862
104.00 0.74 1.27 0.30 315.00 0.00 0.05879 0.17510 0.06501 0.08386 0.12127 0.05189
105.00 0.28 1.10 0.10 185.00 0.00 0.02593 0.05670 0.05328 0.04135 0.09420 0.02666
106.00 0.34 0.92 0.12 188.00 0.00 0.02873 0.06563 0.05433 0.04539 0.07186 0.02163
107.00 1.04 0.91 0.17 191.00 0.00 0.08009 0.24920 0.05702 0.12872 0.07078 0.03166
108.00 0.59 0.90 0.38 296.00 0.00 0.05064 0.14782 0.07008 0.06692 0.06993 0.03089
109.00 0.52 0.80 1.52 261.00 0.00 0.10893 0.43660 0.19409 0.06006 0.05992 0.07821
110.00 0.23 0.77 0.08 276.00 0.00 0.02384 0.04991 0.05251 0.03845 0.05724 0.01588
111.00 0.41 0.68 0.25 288.00 0.00 0.03540 0.08947 0.06189 0.05084 0.04979 0.01809
112.00 3.05 0.67 3.01 339.00 0.00 0.94669 0.99929 0.52541 0.79429 0.04910 0.53659
113.00 3.26 0.52 2.68 297.00 0.00 0.94861 0.99927 0.44090 0.84404 0.03876 0.42408
114.00 1.25 0.52 0.60 243.00 0.00 0.14500 0.49046 0.08597 0.17231 0.03869 0.03081
115.00 0.56 0.51 0.63 349.00 0.00 0.05908 0.18930 0.08889 0.06413 0.03821 0.02233
116.00 0.37 0.47 1.33 342.00 0.00 0.07756 0.30018 0.16616 0.04794 0.03586 0.03840
117.00 0.29 0.45 0.06 278.00 0.00 0.02559 0.05477 0.05151 0.04221 0.03468 0.01007
118.00 0.28 0.41 0.53 316.00 0.00 0.03672 0.10055 0.08083 0.04165 0.03259 0.01519
119.00 0.35 0.38 0.24 317.00 0.00 0.03227 0.07860 0.06139 0.04642 0.03107 0.01125
120.00 0.28 0.38 0.17 488.00 0.00 0.02783 0.06235 0.05792 0.04204 0.03124 0.01017
121.00 0.86 0.37 0.69 350.00 0.00 0.09333 0.32446 0.09398 0.10022 0.03061 0.02248
122.00 0.63 0.37 0.04 339.00 0.00 0.04115 0.10361 0.05069 0.07124 0.03060 0.01043
123.00 0.42 0.35 0.10 320.00 0.00 0.03193 0.07478 0.05366 0.05171 0.02963 0.00968
124.00 0.42 0.35 0.10 334.00 0.00 0.03195 0.07478 0.05370 0.05175 0.02964 0.00969
125.00 0.51 0.33 1.22 361.00 0.00 0.08646 0.32982 0.15132 0.05947 0.02874 0.03025
126.00 0.28 0.32 1.31 335.00 0.00 0.06747 0.25622 0.16330 0.04169 0.02826 0.02909
127.00 0.64 0.31 1.20 355.00 0.00 0.10143 0.38631 0.14867 0.07238 0.02783 0.03072
128.00 0.25 0.27 0.94 398.00 0.00 0.04875 0.15935 0.11836 0.03993 0.02614 0.01848
129.00 0.34 0.25 0.61 413.00 0.00 0.04280 0.12468 0.08754 0.04595 0.02533 0.01349
130.00 0.16 0.24 0.66 345.00 0.00 0.03433 0.09471 0.09138 0.03460 0.02488 0.01275
131.00 0.69 0.23 0.36 312.00 0.00 0.05741 0.17234 0.06882 0.07783 0.02446 0.01210
132.00 0.17 0.23 0.05 339.00 0.00 0.02140 0.04243 0.05118 0.03514 0.02448 0.00682
133.00 0.50 0.22 0.19 320.00 0.00 0.03845 0.09829 0.05852 0.05845 0.02408 0.00913
134.00 0.21 0.21 0.30 334.00 0.00 0.02769 0.06470 0.06507 0.03739 0.02370 0.00870
135.00 0.19 0.21 0.45 361.00 0.00 0.03036 0.07607 0.07514 0.03630 0.02372 0.01003
136.00 0.50 0.19 0.03 335.00 0.00 0.03390 0.07964 0.05019 0.05849 0.02296 0.00744
137.00 0.50 0.19 0.03 355.00 0.00 0.03394 0.07964 0.05024 0.05856 0.02297 0.00744
138.00 0.31 0.19 0.91 398.00 0.00 0.05183 0.17088 0.11520 0.04383 0.02300 0.01646
139.00 0.26 0.18 0.57 413.00 0.00 0.03701 0.10195 0.08433 0.04059 0.02265 0.01124
140.00 0.55 0.17 0.22 345.00 0.00 0.04232 0.11232 0.06031 0.06315 0.02224 0.00898
141.00 0.42 0.17 0.03 312.00 0.00 0.03019 0.06809 0.05013 0.05169 0.02222 0.00693
142.00 0.60 0.15 0.48 339.00 0.00 0.05561 0.16990 0.07721 0.06809 0.02153 0.01162
143.00 0.60 0.15 0.48 320.00 0.00 0.05556 0.16990 0.07713 0.06802 0.02152 0.01162
144.00 0.11 0.14 3.18 334.00 0.00 0.20672 0.78124 0.56831 0.03198 0.02119 0.12436
145.00 0.11 0.14 3.18 361.00 0.00 0.20694 0.78124 0.56869 0.03203 0.02121 0.12445
146.00 0.52 0.13 0.33 335.00 0.00 0.04419 0.12217 0.06696 0.06030 0.02085 0.00932
147.00 0.43 0.13 0.32 355.00 0.00 0.03862 0.10185 0.06640 0.05261 0.02086 0.00882
148.00 0.43 0.13 0.32 398.00 0.00 0.03870 0.10185 0.06655 0.05273 0.02089 0.00884
149.00 0.42 0.13 0.10 413.00 0.00 0.03208 0.07478 0.05393 0.05197 0.02090 0.00704
150.00 0.37 0.13 1.45 345.00 0.00 0.08491 0.33776 0.18386 0.04795 0.02086 0.02662
151.00 0.23 0.13 0.01 312.00 0.00 0.02258 0.04534 0.04916 0.03853 0.02084 0.00582
152.00 0.19 0.13 0.02 213.00 0.00 0.02138 0.04239 0.04938 0.03600 0.02077 0.00575
153.00 0.15 0.13 0.80 239.00 0.00 0.03761 0.11139 0.10341 0.03387 0.02079 0.01240
154.00 0.22 0.12 0.09 223.00 0.00 0.02363 0.04960 0.05287 0.03775 0.02045 0.00617
155.00 0.34 0.11 0.14 315.00 0.00 0.02937 0.06742 0.05576 0.04570 0.02018 0.00682
156.00 0.34 0.11 0.14 185.00 0.00 0.02919 0.06742 0.05538 0.04538 0.02010 0.00679
157.00 0.32 0.11 0.17 188.00 0.00 0.02905 0.06748 0.05701 0.04400 0.02010 0.00693
158.00 0.27 0.11 0.05 191.00 0.00 0.02456 0.05191 0.05078 0.04073 0.02010 0.00598
159.00 0.20 0.11 0.34 296.00 0.00 0.02813 0.06695 0.06747 0.03674 0.02017 0.00778
160.00 0.12 0.11 0.23 261.00 0.00 0.02291 0.04915 0.06062 0.03235 0.02015 0.00667
161.00 0.12 0.11 0.11 276.00 0.00 0.02082 0.04166 0.05406 0.03238 0.02016 0.00591
162.00 0.61 0.10 0.17 288.00 0.00 0.04419 0.11790 0.05731 0.06894 0.01984 0.00793
163.00 0.19 0.10 0.18 339.00 0.00 0.02445 0.05282 0.05802 0.03625 0.01987 0.00649
164.00 0.14 0.10 0.08 126639.00 0.00 0.92180 0.04162 0.98539 0.97939 0.53273 0.25084
165.00 0.21 0.09 0.14 243.00 0.00 0.02426 0.05205 0.05555 0.03720 0.01950 0.00619
166.00 0.18 0.09 1.06 349.00 0.00 0.04839 0.16274 0.13144 0.03571 0.01956 0.01548
167.00 0.13 0.09 0.03 342.00 0.00 0.01988 0.03805 0.05021 0.03301 0.01956 0.00533
168.00 0.13 0.09 0.03 278.00 0.00 0.01981 0.03805 0.05003 0.03290 0.01952 0.00532
169.00 0.12 0.09 0.04 316.00 0.00 0.01972 0.03781 0.05062 0.03245 0.01954 0.00535
170.00 0.12 0.09 0.04 317.00 0.00 0.01972 0.03781 0.05063 0.03245 0.01954 0.00536
171.00 0.11 0.09 0.02 488.00 0.00 0.01929 0.03603 0.05011 0.03225 0.01965 0.00525
172.00 0.39 0.08 0.91 350.00 0.00 0.05785 0.19621 0.11493 0.04946 0.01925 0.01458
173.00 0.15 0.08 0.03 243.00 0.00 0.02036 0.03963 0.04994 0.03388 0.01919 0.00529
174.00 0.25 0.07 0.06 349.00 0.00 0.02424 0.05056 0.05171 0.03983 0.01894 0.00567
175.00 0.20 0.07 0.83 342.00 0.00 0.04156 0.12702 0.10684 0.03683 0.01894 0.01205
176.00 0.19 0.07 0.05 278.00 0.00 0.02197 0.04419 0.05101 0.03613 0.01890 0.00544
177.00 0.23 0.06 0.01 316.00 0.00 0.02259 0.04534 0.04917 0.03854 0.01862 0.00525
178.00 0.17 0.06 0.27 317.00 0.00 0.02550 0.05738 0.06318 0.03509 0.01862 0.00664
179.00 0.35 0.05 0.01 488.00 0.00 0.02709 0.05768 0.04963 0.04685 0.01842 0.00553
180.00 0.24 0.05 0.01 350.00 0.00 0.02295 0.04626 0.04926 0.03921 0.01834 0.00521
181.00 0.21 0.05 0.02 243.00 0.00 0.02204 0.04414 0.04946 0.03720 0.01828 0.00517
182.00 0.20 0.05 0.01 349.00 0.00 0.02166 0.04267 0.04926 0.03684 0.01834 0.00511
183.00 0.15 0.05 0.25 342.00 0.00 0.02441 0.05365 0.06206 0.03406 0.01834 0.00635
184.00 0.10 0.05 0.03 278.00 0.00 0.01897 0.03580 0.05003 0.03138 0.01830 0.00494
185.00 0.08 0.05 0.03 316.00 0.00 0.01846 0.03437 0.05014 0.03048 0.01832 0.00490
186.00 0.37 0.04 0.88 317.00 0.00 0.05487 0.18306 0.11167 0.04787 0.01803 0.01320
187.00 0.33 0.04 1.49 488.00 0.00 0.08342 0.33177 0.19131 0.04543 0.01813 0.02397
188.00 0.18 0.04 0.42 350.00 0.00 0.02921 0.07166 0.07300 0.03571 0.01805 0.00755
189.00 0.94 0.11 0.10 243.00 0.00 0.06634 0.19538 0.05344 0.11192 0.02013 0.00883
190.00 0.30 0.11 0.06 349.00 0.00 0.02606 0.05588 0.05171 0.04304 0.02020 0.00617
191.00 0.18 0.11 0.34 342.00 0.00 0.02739 0.06435 0.06763 0.03570 0.02020 0.00771
192.00 0.46 0.05 0.61 278.00 0.00 0.05039 0.15513 0.08693 0.05486 0.01830 0.01066
193.00 0.33 0.05 0.16 316.00 0.00 0.02942 0.06791 0.05685 0.04500 0.01832 0.00634
194.00 0.12 0.05 2.57 317.00 0.00 0.13811 0.60202 0.41370 0.03245 0.01832 0.06284
195.00 0.25 0.04 0.04 488.00 0.00 0.02402 0.04919 0.05109 0.04013 0.01813 0.00534
196.00 0.16 0.04 0.07 350.00 0.00 0.02145 0.04275 0.05221 0.03461 0.01805 0.00524
197.00 0.09 0.04 0.21 278.00 0.00 0.02160 0.04501 0.05953 0.03090 0.01801 0.00582
198.00 1.15 0.03 0.17 316.00 0.00 0.09349 0.29522 0.05740 0.15096 0.01774 0.00940
199.00 0.19 0.03 0.31 317.00 0.00 0.02710 0.06303 0.06564 0.03621 0.01774 0.00668
200.00 0.10 0.03 0.21 488.00 0.00 0.02215 0.04593 0.06019 0.03175 0.01784 0.00580