Dec 20, 2024
Protocol for Tumorsphere Culture of Cervical Cancer Cell line
This protocol is a draft, published without a DOI.
- Faridah Ismail1
- 1International Islamic University Malaysia
Protocol Citation: Faridah Ismail 2024. Protocol for Tumorsphere Culture of Cervical Cancer Cell line. protocols.io https://protocols.io/view/protocol-for-tumorsphere-culture-of-cervical-cance-dvzj674n
Manuscript citation:
Ismail, F., Zakaria, Y., Isa, M. L. M., & Hassan, N. F. N. (2024). SF1: a standardised fraction of Clinacanthus nutans that inhibits the Stemness properties of Cancer Stem-Like cells derived from Cervical Cancer. Sains Malaysiana, 53(3), 667-679.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 20, 2024
Last Modified: December 20, 2024
Protocol Integer ID: 116491
Keywords: Cervical cancer, SiHa cells, Tumorsphere, Cancer stem cells, Non-adherent 3D culture, study of cervical cancer stem cell, cervical cancer stem cell, siha cervical cancer cell line, cervical cancer cell line this protocol, protocol for tumorsphere culture, cervical cancer cell line, tumorsphere culture, enriching cancer stem, cancer stem, tumorsphere formation, culturing siha, cancer, cervosphere
Abstract
This protocol describes the step-by-step process for culturing SiHa cervical cancer cell lines and enriching cancer stem-like cells (cervospheres) using a non-adherent 3D culture system. The procedure includes preparation of media, culture conditions, tumorsphere formation, and passaging. This method facilitates the study of cervical cancer stem cell (CSC)-like properties in vitro.
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Protocol references
1. Chen, S. F., Chang, Y. C., Nieh, S., Liu, C. L., Yang, C. Y., Lin, Y. S. (2012). Nonadhesive culture system as a model of rapid sphere formation with cancer stem cell properties. PLoS One, 7(2), e31864.
2. Bigoni-Ordóñez, G. D., Ortiz-Sánchez, E., Rosendo-Chalma, P., Valencia-González, H. A., Aceves, C., & García-Carrancá, A. (2018). Molecular iodine inhibits the expression of stemness markers on cancer stem-like cells of established cell lines derived from cervical cancer. BMC Cancer, 18(1), 1–12. https://doi.org/10.2174/1574888x13666181002151330
3. Bahmad, H. F., Cheaito, K., Chalhoub, R. M., Hadadeh, O., Monzer, A., Ballout, F., ... & Abou-Kheir, W. (2018). Sphere-formation assay: three-dimensional in vitro culturing of prostate cancer stem/progenitor sphere-forming cells. Frontiers in oncology, 8, 347.https://doi.org/10.3389/fonc.2018.00347
4. Lee, C. H., Yu, C. C., Wang, B. Y., & Chang, W. W. (2016). Tumorsphere as an effective in vitro platform for screening anti-cancer stem cell drugs. Oncotarget, 7(2), 1215