Dec 09, 2024
Protocol for Investigating Neuronal Communication via Gap Junctions
- Wen Jie Zhu1,
- Wan Hua Li2,
- Hon Cheung Lee1,
- Yong Juan Zhao2
- 1PKU;
- 2CUHKSZ
Protocol Citation: Wen Jie Zhu, Wan Hua Li, Hon Cheung Lee, Yong Juan Zhao 2024. Protocol for Investigating Neuronal Communication via Gap Junctions. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly6jorgx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 09, 2024
Last Modified: December 09, 2024
Protocol Integer ID: 114595
Keywords: gjs intercellular communication among neuron, neuronal communication via gap junction, axonal communication through gj, axonal communication, intercellular communication, investigating neuronal communication, axonal degeneration, sarm1 activation, sensory neuron, sarm1 activity, neuron, cell death, gap junction, targeted therapy, developing targeted therapy, sarm1, drg communication system, cell, understanding of sensory disorder
Abstract
This protocol established a DRG communication system to study axonal communication through GJs. SARM1, an NAD-consuming enzyme, regulates axonal degeneration. Using the SARM1 activity-detecting probe PC11, we found that intercellular communication may regulate SARM1 activation, protecting against axonal degeneration. A related study utilizing this protocol has been published in Cell Death and Disease. This novel approach offers a streamlined method for researches of sensory neuron and GJs intercellular communication among neurons, with potential for advancing our understanding of sensory disorders and developing targeted therapies.
Attachments
Troubleshooting