Nov 22, 2020
Protocol for Freezing in OCT
- 1University of Washington;
- 2Washington University, Saint Louis
- Human BioMolecular Atlas Program (HuBMAP) Method Development CommunityTech. support email: Jeff.spraggins@vanderbilt.edu
Protocol Citation: Shin Lin, Yiing Lin 2020. Protocol for Freezing in OCT. protocols.io https://dx.doi.org/10.17504/protocols.io.bpx7mprn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 22, 2020
Last Modified: November 22, 2020
Protocol Integer ID: 44767
Materials
| CRYO-OCT COMPOUND 4OZ | 25608-930 | VWR | |
| EMBEDDNG MOLD 22X30X20MM CS288 | 15160-270 | VWR | |
| Fisherbrand™ Disposable Base Mold | 22-363-554 | Fisher | |
| PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free | AM9625 | ThermoFisher |
Place tissue sample in culture dish with ice cold PBS.
Cut tissue piece with disposable razor blade.
In mold (choose mold depending on size of tissue), place ice cold OCT to cover the bottom.
Place tissue in mold atop OCT. Fill mold with OCT until tissue is covered.
Place mold atop metal slab bathed in liquid nitrogen until sample is frozen.
Store sample in mold in -80ºC freezer.