Borges A, Radkov A, Thuy-Boun PS. (2022). A workflow to isolate phage DNA and identify nucleosides by HPLC and mass spectrometry. https://doi.org/10.57844/arcadia-1ey9-j808
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: September 19, 2022
Last Modified: January 29, 2024
Collection Integer ID: 70238
Keywords: phage genome modifications with mass spectrometry, phage dna isolation, chemical analysis bacteriophage, phage genome modification, analysis of phage nucleoside, phage nucleoside, bacteriophage, phage growth, phage, mass spectrometry, liquid chromatography, high performance liquid chromatography, degradation by bacterial immune system, performance liquid chromatography, bacterial immune system, dna extraction, bacteria, genome
Abstract
Bacteriophages (phages) are viruses that infect bacteria. Some phages chemically modify their genomes to protect them from degradation by bacterial immune systems. We can detect phage genome modifications with mass spectrometry (MS) and high-performance liquid chromatography (HPLC) of phage nucleosides. This collection contains protocols for the entire process: phage growth and concentration, DNA extraction, and chemical analysis of phage nucleosides. We optimized these protocols using E. coli phage T4 and B. subtilis phage SPO1.
