Nov 24, 2025

Protocol collection - Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor culture

DOI
https://dx.doi.org/10.17504/protocols.io.6qpvrwjd3lmk/v1
  • 1Institute Imagine Paris (24 Bd du Montparnasse, 75015 Paris, France)
  • Hariam Raji: Mechanisms and Therapy of Genetic Brain Diseases
  • michela.deleidi : Mechanisms and Therapy of Genetic Brain Diseases
  • Team Deleidi
Hariam Raji
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DOI: https://dx.doi.org/10.17504/protocols.io.6qpvrwjd3lmk/v1
Collection Citation: Hariam Raji, michela.deleidi 2025. Protocol collection - Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor culture. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvrwjd3lmk/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: November 24, 2025
Last Modified: November 24, 2025
Collection  Integer ID: 233358
Keywords: functional ventral midbrain dopaminergic neuron, human organoid, wnt modulation, bioreactor culture
Abstract
Protocol collection - Engineering functional ventral midbrain dopaminergic neurons in human organoids through WNT modulation and bioreactor culture
Files
Protocol
Name
Dopamine measurement by HPLC
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
MEA Recording of Electrical Activity in Brain Organoids
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Dopamine measurement in midbrain organoids by HPLC
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Treatment of midbrain organoids with αSyn-PFFs
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Human iPSC Culture Protocol
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Immunohistochemistry and Imaging of Human Midbrain Organoid Sections
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Ventral Midbrain Organoids generation from hiPSCs
Version 2
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163
Protocol
Name
Midbrain organoid dissociation for Single-cell transcriptome library preparation and sequencing
Version 1
Created by
Image of Hariam Raji, Institut Imagine Paris - Inserm U1163
Hariam RajiInstitut Imagine Paris - Inserm U1163