Mar 30, 2023

Protocol collection - "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"

  • 1German Center for Neurodegenerative Diseases (DZNE), Tübingen, 72076 Germany
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Collection Citationmichela.deleidi , Maria Jose Perez J., Pascale Baden, Federico Bertoli, Hariam Raji 2023. Protocol collection - "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism". protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr4zr3gmk/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 30, 2023
Last Modified: May 31, 2024
Collection  Integer ID: 79782
Keywords: ASAPCRN, glucocerebrosidase, mitochondria, energy metabolism, deleidi lab, collection of protocol, protocol collection
Abstract
Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
Files
Protocol
Name
Gcase co-immunoprecipitation
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Single cell dissociation of brain organoids
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Plasmid-reprogramming of human fibroblasts
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Sequencing of construct
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Single cell analysis of iPSC-derived midbrain organoids
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Complex I activity assay
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Midbrain-like Organoids generation from hiPSCs
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Expansion microscopy
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Soluble and insoluble A-SYN fractionation
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Mitochondrial complex activity assays
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Differentiation	NPCs	to Dopaminergic/Midbrain Neurons
Name
Differentiation NPCs to Dopaminergic/Midbrain Neurons
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP
Protocol
Name
In vitro GCase activity assay (total cell lysate)
Version 1
Created by
Image of Federico Bertoli, ASAP
Federico BertoliASAP