The protein expression is a process about synthesizing and modification for proteins in livingorganisms with using several laboratory techniques and devices. This technique is generallyused for obtaining protein from the competent cell that is used for carrying plasmids that possessthe protein that we want to express.Protein expression involves two general steps that;• Gene expression; which is continuum that converting DNA to the mRNA that can betranslate into protein.• Protein manufacturing; which is translation process.