Apr 07, 2026
Proliferation and Neurogenesis Analysis
- 1Yale University, USA;
- 2Mohn Research Centre for Regenerative Medicine, Norway
- Brennand Laboratory
Protocol Citation: Novin Balafkan, Kristen Brennand 2026. Proliferation and Neurogenesis Analysis. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9q42pl3e/v1
Manuscript citation:
Garcia, Meilin Fernandez, et al. "Dynamic convergence of neurodevelopmental disorder risk genes across neurodevelopment." bioRxiv (2025): 2024-08.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 07, 2026
Last Modified: April 07, 2026
Protocol Integer ID: 314670
Keywords: Proliferation assay, Ki-67 staining, Neurogenesis, Gliogenesis, FACS, Flow cytometry, neurogenesis analysis this protocol, neurogenesis analysis, neurogenesi, gliogenesi, flow cytometry, evaluation of neural developmental potential, reproducible readout of cell cycle activity, differentiation of human npc, neural developmental potential, cell cycle activity, days of spontaneous differentiation, proliferation, spontaneous differentiation
Funders Acknowledgements:
European Union Horizon 2020 (MSCA)
Grant ID: 101065629
NIH National Institute of Mental Health
Grant ID: R01MH123155
Abstract
This protocol describes a flow cytometry–based method to assess proliferation and differentiation of human NPCs. Proliferation is measured using Ki-67 staining, and neurogenesis and gliogenesis are quantified by surface marker profiling following 25 days of spontaneous differentiation. The expected outcome is a reproducible readout of cell cycle activity and lineage distribution, enabling evaluation of neural developmental potential.
Attachments
