The regenerated transgenic plants (T0) seeds were sown onto Murashige and Skoog Agar (MSA) plates containing 100\u00b5g\/ml Carbenicillin antimicrobial agent, BASTA herbicide (15 \u03bcg\/ml) and allowed to grow. Transgenic lines were selected by BASTA resistant (+). The genotype of T1 plants will be mixtures of homozygous (+\/+), hemizygous (+\/0) and homozygous (-\/-). BASTA resistant T1 seedlings were allowed to grow and T2 seeds were harvested. Then those seeds were grown on MS containing BASTA (15 \u03bcg\/ml) and of them, the ratio of BASTA resistant (+\/+), (+\/-) and susceptible (-\/-) phenotype classes are 3:1.Subsequently, T2- single copy transgenic seeds of above lines and previously created T2 \u2013 single copy transgenic line seeds were grown on MS contained BASTA (15 \u03bcg\/ml). Within the survived plants, ratio of genotype (+\/+) and (+\/-) are 1:2. Those survived T2 seedling were allowed to grow and T3 seeds were harvested from individual plats by avoiding seed cross-contamination between plants.Furthermore, BASTA selection was performed for those T3 seeds and plates that observed all survived seedlings selected as homozygous (+\/+) transgenic lines.