The regenerated transgenic plants (T0) seeds were sown onto Murashige and Skoog Agar (MSA) plates containing 100µg/ml Carbenicillin antimicrobial agent, BASTA herbicide (15 μg/ml) and allowed to grow. Transgenic lines were selected by BASTA resistant (+). The genotype of T1 plants will be mixtures of homozygous (+/+), hemizygous (+/0) and homozygous (-/-). BASTA resistant T1 seedlings were allowed to grow and T2 seeds were harvested. Then those seeds were grown on MS containing BASTA (15 μg/ml) and of them, the ratio of BASTA resistant (+/+), (+/-) and susceptible (-/-) phenotype classes are 3:1.Subsequently, T2- single copy transgenic seeds of above lines and previously created T2 – single copy transgenic line seeds were grown on MS contained BASTA (15 μg/ml). Within the survived plants, ratio of genotype (+/+) and (+/-) are 1:2. Those survived T2 seedling were allowed to grow and T3 seeds were harvested from individual plats by avoiding seed cross-contamination between plants.Furthermore, BASTA selection was performed for those T3 seeds and plates that observed all survived seedlings selected as homozygous (+/+) transgenic lines.