Jun 04, 2019

Public workspaceProduction of magnetic GFP affinity beads using a Camelid anti-GFP nanobody

DOI
dx.doi.org/10.17504/protocols.io.3qqgmvw
  • Nichanok Auevechanichkul1,
  • Ruth Lintermann1,
  • Lennart Wirthmueller1
  • 1Freie Universität Berlin
Lennart Wirthmueller
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DOI: dx.doi.org/10.17504/protocols.io.3qqgmvw
External link: https://figshare.com/articles/Production_of_magnetic_GFP_affinity_beads/6894248
Protocol Citation: Nichanok Auevechanichkul, Ruth Lintermann, Lennart Wirthmueller 2019. Production of magnetic GFP affinity beads using a Camelid anti-GFP nanobody. protocols.io https://dx.doi.org/10.17504/protocols.io.3qqgmvw
Manuscript citation:
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 04, 2019
Last Modified: June 04, 2019
Protocol Integer ID: 24048
Keywords: GFP, YFP, immunoprecipitation, GFP-IP, GFP pulldown, IP-MS, AP-MS, GFP nanobody
Abstract
Our protocol for production of 'home-made' magnetic GFP affinity beads using a Camelid anti-GFP nanobody. These can be used to immuno-purify GFP- or YFP-tagged proteins from cell extracts.