May 14, 2024

Public workspaceProduction of AAV Virus by Iodixanol Gradient Ultracentrifugation V.4

  • Allen Institute for Brain Science1
  • 1Allen Institute
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Protocol CitationAllen Institute for Brain Science 2024. Production of AAV Virus by Iodixanol Gradient Ultracentrifugation. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpr8pdvzp/v4Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 18, 2020
Last Modified: May 14, 2024
Protocol Integer ID: 99806
Keywords: AAV, high purity, high titer
Abstract
This protocol is used to produce AAV of any serotype with high purity and high titer.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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