Cassava is normally propagated using stem cuttings of various sizes. Rooted plantlets are however best for pot experiments, due to the short timeframes of these experiments. Unless large pots (more than 5 L capacity) are used, cassava grown in pots is not usually grown for more than 3 \u2013 4 months. Clear responses to nutrient supply in cassava plants cannot be obtained within such short timeframes with mature cassava stem cuttings because of the large amount of nutrient reserves contained in mature cassava stem cuttings. The nutrient reserves delay the observation of responses to soil nutrient supply (fertiliser application). Responses to other treatments are also probably delayed when mature stem cuttings are planted in pot experiments instead of young succulent, nutrient hungry rooted plantlets. This protocol describes how to produce rooted cassava plantlets or shoots for use in pot experiments. The protocol would not have been developed without guidance from Dr R.H. Howeler. The protocol is also based on a protocol he had already prepared in the Cassava Handbook (see bibliography for citation). Please also refer to it, it is available online for free.