Feb 25, 2025

Public workspaceProcessing plasma from Whole Blood

DOI
https://dx.doi.org/10.17504/protocols.io.ewov1d19kvr2/v1
  • Sara Lucas Del Pozo1,2,
  • Kai-Yin Chau1,2,
  • Jane Macnaughtan1,2,
  • Giuseppe Uras1,2,
  • Roxana Mezabrovschi1,2
  • 1University College London;
  • 2Aligning Science Across Parkinson's
Sofia Koletsi
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DOI: https://dx.doi.org/10.17504/protocols.io.ewov1d19kvr2/v1
Protocol Citation: Sara Lucas Del Pozo, Kai-Yin Chau, Jane Macnaughtan, Giuseppe Uras, Roxana Mezabrovschi 2025. Processing plasma from Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1d19kvr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 06, 2025
Last Modified: February 25, 2025
Protocol Integer ID: 119993
Keywords: ASAPCRN, plasma from whole blood, processing plasma, processing of plasma, whole blood sample, plasma, whole blood, processing, blood, protocol detail, protocol
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-000420
Abstract
This protocol details the processing of plasma from the whole blood sample.
Guidelines
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Troubleshooting
Safety warnings
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Before start
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Procedure
40m
Gently invert the plasma tubes at 180° and back 8-10 times immediately after collection. Allow Duration00:20:00 - Duration00:30:00 to cool down prior to sample processing.

30m
Centrifuge the green cap tube(s) in at Centrifigation1800 x g, Room temperature, 00:10:00 NO BRAKE.

10m
Centrifigation
After centrifugation there should be a clear separation between the red blood cells at the bottom and the plasma at the top.

Use the Pasteur pipette provided or a micropipette to carefully aliquot Amount500 µL of the plasma from the green cap tube (Lithium Heparin) into the 2-3 x 1ml cryovials (Amount500 µL into each tube) labelled as LH-PLA.

Note
Do not to disturb the red blood cell layer. If the sediment is disturbed the centrifugation step must be repeated.

Place plasma samples in Temperature-80 °C .