License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Protocol status: WorkingWe use this protocol and it’s working
Created: June 17, 2020
Last Modified: February 21, 2022
Protocol Integer ID: 38267
Keywords: Restriction endonuclease cloning, cloning into pTXB1, restriction site, Primer desing for cloning, cloning with restriction enzymes, forward primer reverse primer, N-terminal fusion, C-terminal fusion