Apr 28, 2020
Preparing worms for the COPAS (wormsorter)
- 1Imperial College London
- Behavioural Genomics
Protocol Citation: Priota Islam 2020. Preparing worms for the COPAS (wormsorter). protocols.io https://dx.doi.org/10.17504/protocols.io.bfqbjmsn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 28, 2020
Last Modified: October 21, 2020
Protocol Integer ID: 36323
Abstract
This protocol is a guide of how to prepare the worms to be used in the COPAS (wormsorter)
Wash the worms off the plates using few milliliters of M9 and collect in a 15ml falcon tube and fill up till 15ml with M9
Centrifuge the tube at 1500rpm for 2mins followed by removal of the supernatant using a pasteur pipette and addition of another 15ml of M9
Repeat the steps 2 more times
After the final wash remove the supernatant as much as leaving behind 10ml in the falcon tube
Consolidate all the worm liquid in a 50ml tube and dilute if necessary, for the COPAS
Ideally make 2x50ml Falcon tubes
Dispense the worms using the COPAS following the protocol COPAS wormsorter