Oct 17, 2023

Preparing Cholesterol for use in NGM

DOI
https://dx.doi.org/10.17504/protocols.io.36wgq3oe5lk5/v1
  • 1Imperial College London, MRC Laboratory of Medical Sciences
  • Behavioural Genomics
Bonnie Evans
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DOI: https://dx.doi.org/10.17504/protocols.io.36wgq3oe5lk5/v1
Protocol Citation: Bonnie Evans 2023. Preparing Cholesterol for use in NGM. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq3oe5lk5/v1
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 09, 2023
Last Modified: October 17, 2023
Protocol  Integer ID: 89018
Keywords: preparing cholesterol, cholesterol for use, cholesterol, larval stage
Abstract
C. elegans requires cholesterol to progress through the four larval stages.
Materials
CHOLESTEROL-PEG 600 Sigma- C1145-250MG (stored at -20 degrees)
Ethanol absolute (Stored in flammables cupboard)
Prepare 5 mg/ml in Ethanol by resuspending the whole 250mg vial in 50 ml ~100% ethanol.
Filter sterilise using vacuum pump.
Store at 4 degrees protected from the light