Mar 12, 2023

Public workspacePreparing Agarose Gel

DOI
https://dx.doi.org/10.17504/protocols.io.kqdg391q1g25/v1
  • alyssa.jeng 1
  • 1University of Colorado
alyssa.jeng
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DOI: https://dx.doi.org/10.17504/protocols.io.kqdg391q1g25/v1
Protocol Citation: alyssa.jeng 2023. Preparing Agarose Gel. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg391q1g25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 11, 2023
Last Modified: March 12, 2023
Protocol Integer ID: 78544
Abstract
N/A
Troubleshooting
Materials
  • 1X TAE Buffer
  • SYBRsafe
  • Agarose powder
  • Weigh boat
  • 500mL Erlenmeyer flask
  • Gel Electrophoresis tray
  • Gel Electrophoresis comb
  • Tape
Procedure
Tape up the gel electrophoresis tray and add well comb
Add 200mL of 1X TAE buffer to the Erlenmeyer flask
Weigh the right amount of agarose powder - depends on the expected PCR products: smaller than 500 bp: 2% agarose gel – 4 gr/200mL; bigger than 500 bp: 1% agarose gel – 2 gr/200mL).
Microwave for 2-3 minutes
Carefully remove flask from microwave and add 20uL of SYBRsafe to the gel solution. Swirl to mix.
Slowly pour the mixture in to the electrophoresis tray
Let cool for at least 30 minutes (no longer than 2 hours)
Clean the Erlenmeyer flask to remove excess agarose gel