Jul 24, 2019

Public workspacePrepare NGM plates for nematode, with peptone, without fungizone V.2

DOI
dx.doi.org/10.17504/protocols.io.5smg6c6
  • Cancer Research UK / Wellcome Gurdon Institute media kitchen1
  • 1Wellcome Trust / Cancer Research UK Gurdon Institute
Cristian Riccio
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DOI: dx.doi.org/10.17504/protocols.io.5smg6c6
Protocol Citation: Cancer Research UK / Wellcome Gurdon Institute media kitchen 2019. Prepare NGM plates for nematode, with peptone, without fungizone. protocols.io https://dx.doi.org/10.17504/protocols.io.5smg6c6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 24, 2019
Last Modified: July 24, 2019
Protocol Integer ID: 26157
Abstract
Prepare NGM plates for nematodes, with peptone, without fungizone
Materials
MATERIALS
Reagentdouble distilled water (ddH2O)
ReagentSodium chloride meets analytical specification of Ph.Eur Fisher ChemicalFisher ScientificCatalog #S/3160/65
ReagentSYCHEM autoclaveSyschem
ReagentAgarFormediumCatalog #AGA02
Reagent Minisart filters pore size 0.2 μm Merck MilliporeSigma (Sigma-Aldrich)Catalog #16534K
ReagentBacto™ PeptoneThermo Fisher ScientificCatalog #211677
Safety warnings
Make sure you know how to use the autoclave before starting this protocol.
Download NGM_agar_1L.xlsNGM_agar_1L.xls

Ingredients Quantity
NaCl 3g
bacto peptone 2.5g
Agar 17g
Double distilled H2O972ml
Measure 972ml  double distilled H2O and put in a 1L duran bottle with a magnetic flea
Add 3g NaCl, 2.5g bacto peptone and stir.
Stir until all solutes are dissolved then add the agar.
Leave magnetic flea in bottle.
Label, date and autoclave